Sequence analysis of the gene encoding H antigen in Escherichia coli isolated from food in Morocco.
Identifieur interne : 000104 ( Main/Exploration ); précédent : 000103; suivant : 000105Sequence analysis of the gene encoding H antigen in Escherichia coli isolated from food in Morocco.
Auteurs : Samira Badri [Maroc] ; Aziz Fassouane ; Ingrid Filliol ; Mohammed Hassar ; Nozha CohenSource :
- Journal of microbiology (Seoul, Korea) [ 1976-3794 ] ; 2010.
Descripteurs français
- KwdFr :
- ADN bactérien (composition chimique), ADN bactérien (génétique), Analyse de regroupements (MeSH), Analyse de séquence (MeSH), Antigènes bactériens (génétique), Données de séquences moléculaires (MeSH), Escherichia coli (classification), Escherichia coli (génétique), Escherichia coli (isolement et purification), Flagelline (MeSH), Génotype (MeSH), Maroc (MeSH), Microbiologie alimentaire (MeSH), Polymorphisme de restriction (MeSH), Profilage d'ADN (méthodes), Protéines Escherichia coli (génétique), Réaction de polymérisation en chaîne (méthodes), Techniques de typage bactérien (méthodes), Type II site-specific deoxyribonuclease (métabolisme).
- MESH :
- composition chimique : ADN bactérien, Escherichia coli.
- génétique : ADN bactérien, Antigènes bactériens, Escherichia coli, Protéines Escherichia coli.
- isolement et purification : Escherichia coli.
- métabolisme : Type II site-specific deoxyribonuclease.
- méthodes : Profilage d'ADN, Réaction de polymérisation en chaîne, Techniques de typage bactérien.
- Analyse de regroupements, Analyse de séquence, Données de séquences moléculaires, Flagelline, Génotype, Maroc, Microbiologie alimentaire, Polymorphisme de restriction.
- Wicri :
- geographic : Maroc.
English descriptors
- KwdEn :
- Antigens, Bacterial (genetics), Bacterial Typing Techniques (methods), Cluster Analysis (MeSH), DNA Fingerprinting (methods), DNA, Bacterial (chemistry), DNA, Bacterial (genetics), Deoxyribonucleases, Type II Site-Specific (metabolism), Escherichia coli (classification), Escherichia coli (genetics), Escherichia coli (isolation & purification), Escherichia coli Proteins (genetics), Flagellin (MeSH), Food Microbiology (MeSH), Genotype (MeSH), Molecular Sequence Data (MeSH), Morocco (MeSH), Polymerase Chain Reaction (methods), Polymorphism, Restriction Fragment Length (MeSH), Sequence Analysis (MeSH).
- MESH :
- chemical , chemistry : DNA, Bacterial.
- chemical , genetics : Antigens, Bacterial, DNA, Bacterial, Escherichia coli Proteins.
- chemical , metabolism : Deoxyribonucleases, Type II Site-Specific.
- geographic : Morocco.
- classification : Escherichia coli.
- genetics : Escherichia coli.
- isolation & purification : Escherichia coli.
- methods : Bacterial Typing Techniques, DNA Fingerprinting, Polymerase Chain Reaction.
- Cluster Analysis, Flagellin, Food Microbiology, Genotype, Molecular Sequence Data, Polymorphism, Restriction Fragment Length, Sequence Analysis.
Abstract
In order to develop other molecular method useful for typing of motile and non motile Escherichia coli strains, a total of 207 strains of E. coli (133 reference strains, 74 food strains) were characterized by analysis of sequences of their amplified flagellin-encoding (fliC) gene products. The collection of reference strains was used for database building of fliC gene sequences. Application of this identification system to 74 E. coli food isolates revealed a reproducible and clear cut classification with very good correlation to results obtained by HhaI restriction of the amplified flagellin gene. The proposed determination of fliC sequences variations should be helpful for epidemiological studies.
DOI: 10.1007/s12275-010-9182-1
PubMed: 20437150
Affiliations:
Links toward previous steps (curation, corpus...)
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- to stream PubMed, to step Checkpoint: 000701
- to stream Main, to step Merge: 000104
- to stream Main, to step Curation: 000104
Le document en format XML
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first="Ingrid" last="Filliol">Ingrid Filliol</name></author><author><name sortKey="Hassar, Mohammed" sort="Hassar, Mohammed" uniqKey="Hassar M" first="Mohammed" last="Hassar">Mohammed Hassar</name></author><author><name sortKey="Cohen, Nozha" sort="Cohen, Nozha" uniqKey="Cohen N" first="Nozha" last="Cohen">Nozha Cohen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20437150</idno><idno type="pmid">20437150</idno><idno type="doi">10.1007/s12275-010-9182-1</idno><idno type="wicri:Area/PubMed/Corpus">000713</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000713</idno><idno type="wicri:Area/PubMed/Curation">000709</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000709</idno><idno type="wicri:Area/PubMed/Checkpoint">000701</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000701</idno><idno 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(chemistry)</term><term>DNA, Bacterial (genetics)</term><term>Deoxyribonucleases, Type II Site-Specific (metabolism)</term><term>Escherichia coli (classification)</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (isolation & purification)</term><term>Escherichia coli Proteins (genetics)</term><term>Flagellin (MeSH)</term><term>Food Microbiology (MeSH)</term><term>Genotype (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Morocco (MeSH)</term><term>Polymerase Chain Reaction (methods)</term><term>Polymorphism, Restriction Fragment Length (MeSH)</term><term>Sequence Analysis (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (composition chimique)</term><term>ADN bactérien (génétique)</term><term>Analyse de regroupements (MeSH)</term><term>Analyse de séquence (MeSH)</term><term>Antigènes bactériens (génétique)</term><term>Données de séquences moléculaires (MeSH)</term><term>Escherichia coli (classification)</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (isolement et purification)</term><term>Flagelline (MeSH)</term><term>Génotype (MeSH)</term><term>Maroc (MeSH)</term><term>Microbiologie alimentaire (MeSH)</term><term>Polymorphisme de restriction (MeSH)</term><term>Profilage d'ADN (méthodes)</term><term>Protéines Escherichia coli (génétique)</term><term>Réaction de polymérisation en chaîne (méthodes)</term><term>Techniques de typage bactérien (méthodes)</term><term>Type II site-specific deoxyribonuclease (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Bacterial</term><term>DNA, Bacterial</term><term>Escherichia coli Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Deoxyribonucleases, Type II 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type="geographic" xml:lang="fr"><term>Maroc</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In order to develop other molecular method useful for typing of motile and non motile Escherichia coli strains, a total of 207 strains of E. coli (133 reference strains, 74 food strains) were characterized by analysis of sequences of their amplified flagellin-encoding (fliC) gene products. The collection of reference strains was used for database building of fliC gene sequences. Application of this identification system to 74 E. coli food isolates revealed a reproducible and clear cut classification with very good correlation to results obtained by HhaI restriction of the amplified flagellin gene. The proposed determination of fliC sequences variations should be helpful for epidemiological studies.</div></front></TEI><affiliations><list><country><li>Maroc</li></country></list><tree><noCountry><name sortKey="Cohen, Nozha" sort="Cohen, Nozha" uniqKey="Cohen N" first="Nozha" last="Cohen">Nozha Cohen</name><name sortKey="Fassouane, Aziz" sort="Fassouane, Aziz" uniqKey="Fassouane A" first="Aziz" last="Fassouane">Aziz Fassouane</name><name sortKey="Filliol, Ingrid" sort="Filliol, Ingrid" uniqKey="Filliol I" first="Ingrid" last="Filliol">Ingrid Filliol</name><name sortKey="Hassar, Mohammed" sort="Hassar, Mohammed" uniqKey="Hassar M" first="Mohammed" last="Hassar">Mohammed Hassar</name></noCountry><country name="Maroc"><noRegion><name sortKey="Badri, Samira" sort="Badri, Samira" uniqKey="Badri S" first="Samira" last="Badri">Samira Badri</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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